JPS6231909B2 - - Google Patents
Info
- Publication number
- JPS6231909B2 JPS6231909B2 JP54038418A JP3841879A JPS6231909B2 JP S6231909 B2 JPS6231909 B2 JP S6231909B2 JP 54038418 A JP54038418 A JP 54038418A JP 3841879 A JP3841879 A JP 3841879A JP S6231909 B2 JPS6231909 B2 JP S6231909B2
- Authority
- JP
- Japan
- Prior art keywords
- serum
- cells
- medium
- culture
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000002966 serum Anatomy 0.000 claims description 14
- 239000000758 substrate Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 19
- 239000002609 medium Substances 0.000 description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 244000309466 calf Species 0.000 description 4
- 239000012510 hollow fiber Substances 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000012679 serum free medium Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 235000015115 caffè latte Nutrition 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000003953 foreskin Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3841879A JPS55131387A (en) | 1979-04-02 | 1979-04-02 | Cultivation of cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3841879A JPS55131387A (en) | 1979-04-02 | 1979-04-02 | Cultivation of cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS55131387A JPS55131387A (en) | 1980-10-13 |
| JPS6231909B2 true JPS6231909B2 (en]) | 1987-07-10 |
Family
ID=12524755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3841879A Granted JPS55131387A (en) | 1979-04-02 | 1979-04-02 | Cultivation of cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55131387A (en]) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100437081B1 (ko) * | 2001-09-12 | 2004-06-23 | 주식회사 엘지생명과학 | 이산화탄소 농도를 조절하여 재조합 단백질의 생산성을증가시키는 동물세포의 배양방법 |
-
1979
- 1979-04-02 JP JP3841879A patent/JPS55131387A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS55131387A (en) | 1980-10-13 |
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